Lesson 7. Monitoring Persons On and Off HBV Therapy

Chronic hepatitis B virus (HBV) infection is a dynamic disease process that reflects the interplay between the virus and the host immune response (Figure 1).[1,2,3] Ultimately, it is the ongoing cycles of host-mediated immune response to the virus and associated inflammatory activity, or flares, that are thought to drive hepatocyte injury. Each cycle of chronic necroinflammation and inefficient clearance of the virus can contribute to the development of liver fibrosis and cumulative risk of cirrhosis.[2] Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), and HBV DNA levels are, therefore, the cornerstone of HBV disease monitoring, as they provide a window into this dysfunctional interaction between host and virus. Regularly assessing these biomarkers over time provides us with the most readily available guide to assess disease activity and decide when antiviral therapy is indicated. Monitoring while on treatment is recommended for two reasons: (1) to evaluate for any medication-related complications to ensure the ongoing safety of the treatment, and (2) to assess the effectiveness of treatment and to detect treatment failure if it occurs. This lesson will review the key elements of monitoring persons with chronic HBV, including persons who are not on therapy, persons who are receiving treatment, and persons who discontinue treatment.

Persons with chronic HBV who do not meet the criteria for treatment initiation should continue to be monitored with serum ALT and HBV DNA levels at regular intervals to determine if they are transitioning into a more immune active state that might warrant antiviral therapy.[1,4,5] For a detailed discussion of indications for initiating HBV therapy, see the lesson When to Initiate HBV Treatment. In general, monitoring of ALT levels and HBV DNA is performed every 3 to 6 months in these untreated patients. Emphasizing the importance of regularly monitoring disease activity is a crucial aspect of counseling patients with chronic HBV and managing their expectations when they first seek care.

AASLD and AASLD/IDSA Hepatitis B Guidance

Recommendations for laboratory monitoring in chronic HBV infection are addressed in detail in the 2018 AASLD Hepatitis B Guidance and to a lesser extent in the 2025 AASLD/IDSA HBV Treatment Guideline. These guidelines provide laboratory monitoring recommendations that vary depending on hepatitis B e antigen (HBeAg) status; the ALT and HBV DNA thresholds used for clinical decision-making can vary depending on this HBeAg status.[1,6] The following summarizes recommendations for monitoring in persons with a positive HBsAg who do not have cirrhosis and are not on treatment. The upper limit of normal (ULN) is considered 25 U/L for women and 35 U/L for men, per this document.[1]

• HBeAg Positive: In general, monitoring should consist of the HBV DNA levels and ALT levels every 3–6 months. In addition, persons with normal ALT levels and HBV DNA levels greater than 20,000 IU/mL should have HBeAg checked every 6–12 months. More frequent monitoring is indicated if the ALT level is elevated at baseline, or the ALT becomes elevated during monitoring. If the ALT remains less than the ULN during the first year of monitoring, then the frequency of monitoring can be reduced to every 6 months.
• HBeAg Negative: For the first year of care for hepatitis B, monitoring of ALT and HBV DNA every 3 months and HBsAg once yearly is advised to determine if the patient is in an inactive or more active phase of chronic liver disease. More frequent monitoring (and evaluation of potential causes) is indicated if the ALT level remains elevated during monitoring. If the patient is stable with a consistently normal-range ALT for a minimum of 12 months, subsequent ALT testing can be extended to every 6 months.

Hepatitis B Primary Care Guidance

For persons with chronic hepatitis B who are not on treatment, the 2025 HBV Primary Care Workgroup Guidance recommends laboratory monitoring with serum ALT and HBV DNA levels on average every 6 months (range every 3–12 months), and this does not vary by HBeAg status.[7] More frequent testing is indicated if there is persistent ALT elevation above the upper limit of normal (greater than 25 IU/L in women and greater than 35 IU/L in men), which might indicate a transition to an immune-active phase that would warrant therapy.[7]

Monitoring for Changes in HBV Antigen/Antibody Status

The loss of HBeAg (in patients who are HBeAg-positive at baseline) or the loss of HBsAg represents key immunologic milestones in patients with chronic HBV. Annual testing for HBsAg should be considered in patients who are HBeAg negative and have consistently normal ALT and low (<2,000 IU/mL) HBV DNA levels (e.g., inactive carriers), as HBsAg loss can occur spontaneously in these individuals.[1] Annual testing for HBeAg and anti-HBe should be considered in persons with immune-tolerant HBV infection (elevated HBV DNA but normal ALT), as these individuals may spontaneously lose their HBeAg-positive status.[1] Nevertheless, the cost-benefit utility of routine HBeAg, anti-HBe, and HBsAg serologic monitoring in untreated patients is unclear, particularly as HBsAg loss is considered a rare event, and HBeAg loss in patients not meeting HBV treatment criteria is unlikely to change management. Serologic testing of hepatitis B e antigen/antibody or surface antigen/antibody is considered optional in primary care settings.

Liver Disease Staging

The 2018 AASLD Hepatitis B Guidance and 2025 AASLD/IDSA HBV Treatment Guideline note that liver disease severity should be assessed as part of the initial evaluation of patients with HBV.[1,6] Assessment of liver disease severity should include a baseline laboratory evaluation consisting of complete blood count (CBC) with platelet count, hepatic aminotransferases (ALT and AST), alkaline phosphatase, bilirubin, serum albumin, and prothrombin time at baseline.[1] In persons with viral hepatitis, ALT typically exceeds AST, but with advanced fibrosis, this ratio typically reverses. In addition to basic laboratory testing, there are several methods for staging liver fibrosis, which can be done to further evaluate liver disease in patients with HBV. When monitoring individuals not yet on therapy, determination of liver disease severity is key to making treatment and management& decisions. Although liver biopsy is the gold standard for staging liver fibrosis, non-invasive methods, including calculating a FIB-4 and/or performing transient elastography, have& emerged as safer and more convenient options, and are endorsed by major guidelines.[1,6] When monitoring off of therapy, the 2018 AASLD Hepatitis B Guidance recommends that liver biopsy, or an alternative form of fibrosis assessment, be considered in patients with persistently elevated ALT that is borderline normal or mildly elevated (range of 1 to 2 times upper limit of normal: 25 IU/L in women, 35 IU/L in men), particularly if they acquired HBV at a very young age (as can occur in countries where perinatal acquisition is the most common route for HBV acquisition) and if they are older than 40 years of age.[1] Patients who are found to have at least moderate to severe inflammation (Metavir score A3 or higher) or fibrosis (Metavir score F2 or higher) are candidates for antiviral therapy.

• APRI and FIB-4: The AST-platelet ratio index (APRI) and Fibrosis-4 (FIB-4) index are scores that estimate the degree of underlying fibrosis in patients with viral hepatitis based on routinely available blood tests (see APRI and FIB-4 calculators). FIB-4 has been shown to have reasonable performance in differentiating mild (stages 0–1) from more advanced (stages 3–4) fibrosis in chronic HBV.[8,9] It should be noted, however, that these standard biomarkers are not sufficiently sensitive to detect advanced liver disease, particularly in patients with chronic HBV on antiviral therapy.
• Elastography: Vibration-controlled transient elastography (VCTE or FibroScan) is a noninvasive office-based test that is performed using an ultrasound transducer probe to measure shear wave velocity, which correlates directly with liver stiffness. It has been shown to have reasonably good diagnostic performance for the detection of advanced fibrosis in a variety of patient settings, including HBV, and it is the preferred alternative method for assessing liver stiffness in patients with HBV when liver biopsy is not performed.[1,6,10] Magnetic resonance elastography uses image capture of the shear waves to estimate liver stiffness and has emerged as another method of assessing disease with high accuracy in chronic HBV.[11]

• Liver Biopsy: Liver biopsy has been the gold standard for assessing liver disease severity in chronic HBV and is the only means by which we can assess both inflammatory activity and fibrosis. The risk of complications such as intrahepatic bleeding or biliary injury is low but can be up to 1.1% in patients with advanced liver disease.[12] Biopsy can also result in misclassification, particularly if there is undersampling of liver tissue. Liver biopsy, however, can detect significant liver inflammation or fibrosis that is not always reflected in serum biomarkers and is therefore recommended in a subset of patients with chronic hepatitis B.[5]

Hepatocellular Carcinoma Screening

Hepatocellular carcinoma (HCC) screening is part of HBV monitoring and should occur when indicated, regardless of HBV treatment status.[1,4,13] For additional information on indications for HCC screening, please see the lesson Screening for Hepatocellular Carcinoma.

Safety Monitoring with Oral Antiviral Therapy

Oral antiviral therapy for HBV, using a first-line nucleoside analogue (entecavir) or nucleotide analogue (tenofovir alafenamide [TAF] or tenofovir DF [TDF]), is generally considered safe, with minimal laboratory monitoring for safety needed, aside from renal safety monitoring in persons receiving tenofovir DF. Tenofovir DF has the potential to cause nephrotoxicity, and most experts would avoid its use in persons with preexisting renal insufficiency (estimated creatine clearance less than 60 mL/min) and in persons with clinical features that are associated with a higher renal risk. In contrast, tenofovir alafenamide can be safely used for the treatment of HBV in persons with a creatinine clearance of greater than or equal to 15 mL/min or in persons receiving hemodialysis. Entecavir can be safely used in persons with renal insufficiency, but it requires a dose adjustment if the estimated creatinine clearance is less than 50 mL/min.

• Baseline Laboratory Studies: Prior to starting oral antiviral therapy, the following baseline laboratory studies are recommended:
  • Complete blood count (CBC)
  • Hepatic function panel that includes total bilirubin, ALT, AST, albumin and alkaline phosphatase
  • Renal function assessment should include (1) a serum creatine to estimate creatinine clearance and calculate the glomerular filtration rate (GFR) and (2) evaluation of clinical risk factors for renal insufficiency. Preexisting renal insufficiency (GFR less than 60 mL/min) from any cause, including extrahepatic HBV-associated renal disease, would indicate an increased risk of tenofovir DF nephrotoxicity.
  • HBV DNA levels with a highly sensitive quantitative assay should be ordered, as well as a hepatitis B e antigen (HBeAg) and hepatitis B e antibody (anti-HBe) if the status is not known.
  • Baseline HIV test is recommended before treatment initiation—if the individual had undiagnosed HIV, use of monotherapy for HBV with entecavir, tenofovir alafenamide, or tenofovir DF would likely result in HIV drug resistance, due to the intrinsic HIV activity of these medications and the need to treat HIV with combination antiretroviral therapy.
  • All patients with chronic HBV should also be screened for hepatitis C virus (HCV), given shared risk factors and faster progression of liver fibrosis in coinfection. Screening for HCV is done via the detection of HCV antibodies.
• Evaluation for Renal Risk for Patients on Tenofovir DF: An assessment of renal risk should be made on an ongoing basis for those receiving TDF. The clinical factors associated with an increased risk for renal disease with TDF include decompensated cirrhosis, low body weight, older age, and the use of concurrent nephrotoxic medications. For persons receiving tenofovir DF, monitoring includes serum creatinine, serum phosphorus, and urinalysis to assess urine glucose and proteinuria—the recommended interval of testing is at least once annually and more frequently in patients at risk for renal injury or with preexisting renal impairment.[1] In cases of known or suspected TDF-associated nephrotoxicity, TDF should be discontinued and substituted with either TAF or entecavir, with appropriate consideration of prior treatment experience and drug resistance, before making this selection.
• Monitoring for Lactic Acidosis: The development of lactic acidosis is a rare complication of nucleoside/nucleotide therapy; this has been seen with both tenofovir DF and entecavir in patients with chronic HBV.[14,15] The mechanism for this is thought to be the drug’s inhibition of mitochondrial polymerase gamma inside the liver and muscle. It is a potentially life-threatening complication that can manifest insidiously with non-specific symptoms such as fatigue, nausea, malaise, or abdominal discomfort. Risk factors for this complication include decompensated cirrhosis, particularly with a model for end-stage liver disease (MELD) score greater than 20, and concurrent use of medications with additive mitochondrial toxicity are risk factors.[15] Routine laboratory monitoring for lactic acidosis is not necessary but assessment of the anion gap and testing of lactic acid level is warranted in a symptomatic patient with advanced liver disease.

Safety Monitoring with Peginterferon Therapy

Peginterferon-based therapy requires more frequent laboratory monitoring for safety and efficacy than oral antiviral therapy due to its unique set of toxicities. Adverse events with peginterferon can be diverse and range from initial flu-like symptoms to fatigue, nausea, diarrhea, anorexia with or without weight loss, emotional lability and depression, bone marrow suppression, and autoimmune phenomena such as thyroiditis. All persons receiving peginterferon should be monitored for these complications throughout the 48 weeks of therapy. In some individuals, these adverse effects can be severe enough to warrant dose reduction or discontinuation of therapy.

• Baseline Laboratory Studies: At baseline, a CBC, liver panel, renal function panel, HBV DNA level, HBeAg, anti-HBe, and thyroid-stimulating hormone (TSH) level should be sent.
• Laboratory Monitoring on Treatment: Serum ALT should be assessed every 1-3 months during treatment to assess for hepatic flares since these often occur during interferon-based therapy for HBV. Laboratory monitoring should also include TSH every 3 months and a CBC with differential every 1-2 months, with the exact frequency determined by changes observed.
• Additional Monitoring on Therapy: Patients on peginterferon should be monitored at each visit for symptomatic anemia, as well as other complications, including neuropsychiatric disturbances in sleep or mood, infections, or autoimmune complications.

An understanding of virologic responses is helpful to assess treatment effectiveness in the context of both oral antiviral therapy and peginterferon-based therapy. The following are general definitions of terms describing the spectrum of treatment responses.[1,4,5,13]

Biochemical Responses (Based on ALT Levels)

• Biochemical Response: Persons on HBV treatment are considered to have a biochemical response to treatment if normalization of serum ALT level occurs while on treatment.
• Clinical Relapse: This occurs when a viral relapse (see definition below) is accompanied by or followed shortly by a rise in serum ALT to greater than 2 times the baseline ALT.
• Hepatitis Flare: A hepatitis flare is defined as an ALT increase of 3 times the baseline level and greater than 100 U/L.

Serologic Responses

A serologic response in a person with HBV who is receiving treatment can be described based on the dynamics of (1) HBeAg and anti-HBe or (2) HBsAg and anti-HBs (Figure 2).

Virologic Responses (Based on HBV DNA)

• Virologic Response (with Oral Antiviral Therapy): For persons receiving oral nucleoside analogue HBV therapy, a virologic response is defined as an undetectable HBV DNA level using a sensitive polymerase chain reaction (PCR)-based assay for viral quantitation, where the lower limit of detection is 10-12 IU/mL (Figure 3). Some expert panels suggest using a threshold of less than 60 IU/mL to signify a virologic response with oral nucleoside analogue therapy.
• Virologic Response (with Peginterferon Therapy): For persons receiving peginterferon for treatment of HBV, a virologic response is defined as the achievement of an HBV DNA level below 2,000 IU/mL (Figure 4).[16] Some experts have used 20,000 IU/mL as a higher threshold to define virologic response with peginterferon therapy.
• Primary Virologic Response: A decline in HBV DNA of greater than 1 log10 IU/mL by 12 weeks of starting oral antiviral therapy (Figure 5).
• Primary Virologic Nonresponse: This is defined by a decline of HBV DNA level that is less than 1 log10 IU/mL at 12 weeks on oral antiviral therapy (Figure 6).
• Partial Virologic Response: This occurs when the decline of HBV DNA level is greater than 1 log10 IU/mL but still detectable at 24 weeks of oral antiviral therapy (in a person who is adherent with therapy) (Figure 7).
• Virologic Breakthrough: An increase of HBV DNA levels to greater than 1 log10 IU/mL compared with the lowest (nadir) HBV DNA level (Figure 8).
• HBV Reactivation: (1) a 2 log10 (100-fold) or more increase in HBV DNA level compared to baseline or (2) an HBV DNA level of at least 3 log10 (1,000) IU/mL in a patient previously undetectable (Figure 9).
• Viral Relapse: This refers to the emergence of an HBV DNA level greater than 2,000 IU/mL after stopping therapy in patients with a virologic response (Figure 10).
• Sustained Off-Treatment Virologic Response with Oral Therapy: For persons who discontinue oral antiviral therapy, this is defined as the presence of HBV DNA levels less than 2,000 IU/mL for at least 12 months after stopping therapy; this response is most likely to occur only after multiple years of oral therapy (Figure 11).
• Sustained Off-Treatment Virologic Response with Peginterferon Therapy: This describes the presence of HBV DNA levels less than 2,000 IU/mL for at least 12 months after stopping peginterferon treatment; the typical HBV treatment course with peginterferon therapy is 48 weeks (Figure 12).

Monitoring Treatment Response with Oral Antiviral Therapy

The cornerstone of monitoring the treatment responses in patients taking oral antiviral therapy is tracking the HBV DNA level. The degree to which HBV viral suppression is achieved and sustained during therapy has been shown to be a key predictor of clinical outcomes.[17,18] This is not surprising, given our understanding of HBV replication and the major role it plays in driving liver disease progression (both decompensated liver disease and hepatocellular carcinoma).[19,20] Ongoing monitoring of HBV DNA levels is needed to evaluate the initial treatment response and ensure early detection of virologic breakthrough and treatment failure. Treatment-emergent drug resistance can occur with oral antiviral therapy, although this is rare with the use of any of the three preferred oral antiviral therapy options (TDF, TAF, and entecavir).

Monitoring HBV DNA Levels on Therapy

Most HBV guidelines recommend that HBV DNA quantification be ordered at baseline prior to treatment, as well as at months 3 and 6 after initiating therapy.[1,4,5] Most persons who have consistent adherence to their treatment will achieve a decline in HBV DNA of greater than 1 log10 IU/mL by 12 weeks (primary virologic response) on one of the first-line nucleoside analogue agents (entecavir, tenofovir alafenamide, or tenofovir DF). If they do not, additional counseling on adherence and exploration of barriers to treatment is indicated. Following initiation of HBV therapy, the magnitude and timing of HBV viral decline can vary significantly. The degree and rapidity of the HBV DNA decline often depend on the person’s immunologic response against HBV, with those who are more immune active (i.e., higher baseline ALT) and less immune tolerant more likely to experience an efficient decline in HBV DNA levels.[21] Once a primary virologic response has been achieved, HBV DNA levels should continue to be monitored every 3 to 6 months.

• Time to Achieve Virologic Response: A virologic response, or achievement of complete HBV suppression to undetectable levels, is an important endpoint in nucleoside analogue therapy but can be delayed in many patients and often takes longer than 48 weeks, primarily due to the slower kinetics of HBV viral decline (compared with treatment-related declines in viral levels with HIV or HCV therapy).
• Approach to Persistent Viremia: Persistent viremia is defined as a leveling or plateau in the decline of HBV DNA and/or failure to achieve an undetectable HBV DNA level by 96 weeks of therapy. Persistent low-level viremia is an HBV DNA level that decreases to less than 2,000 IU/ml but remains detectable at 96 weeks. Persistent low-level viremia may be associated with adverse clinical outcomes, namely accelerated fibrosis progression and increased risk for hepatocellular carcinoma.[22,23] The AASLD does not recommend augmenting or changing therapy in such cases, even in the setting of an elevated ALT level.[1]
• Approach to Virologic Breakthrough: Virologic breakthrough is defined as a 10-fold (1 log10 IU/ml) or greater increase in HBV DNA level in persons on treatment. This finding needs to be confirmed with a repeat HBV DNA level. This most often indicates a lapse in medication adherence or an unanticipated medication interruption. Thus, the finding of a virologic breakthrough should first lead to an adherence evaluation, including a thorough review of pharmacy refill records, before pursuing further testing for HBV drug resistance. If good adherence is likely, then a virologic breakthrough should be considered. A virologic breakthrough can indicate the emergence of antiviral resistance mutations and, if left unchecked, can lead to the acquisition of additional mutations, further HBV viral replication, and, in some cases, hepatitis flares and hepatic decompensation.[24]

HBV Resistance Testing

In most settings, drug resistance is confirmed by sending an HBV genotypic resistance assay using consensus-based sequencing (similar to that used for HIV resistance testing) that is used to detect viral subpopulations bearing amino acid substitutions (mutations) in the reverse transcriptase region of the HBV genome known to confer drug resistance. The probability of resistance is influenced by a variety of clinical factors, including pretreatment HBV DNA level, prior treatment exposure, duration and potency of antiviral therapy, and the genetic barrier for resistance of the antiviral agent. The rates of HBV resistance are highest for lamivudine (65-70% at 4-5 years), and adefovir (29% at 5 years), and lowest for entecavir in the absence of lamivudine resistance (2% at 5 years) and tenofovir (3% at 5 years) (Figure 13).[4,25,26] Management of drug resistance is a separate topic not covered in this discussion and should be done with expert consultation.

Monitoring Biochemical Response

A hepatic function panel (serum ALT, AST with or without total bilirubin, albumin, and alkaline phosphatase) is generally performed concurrently with the HBV DNA monitoring in persons on oral antiviral therapy. In most cases, the beginning of a biochemical response, as evidenced by the normalization of ALT and AST elevation (typically <25 U/L in women, <35 U/L in men), can be seen as early as a few months after the initiation of nucleoside analogue therapy in patients with immune-active disease, typically coincident with the decline in HBV DNA level.

Monitoring Serologic Response

• HBeAg Loss and anti-HBe Seroconversion: Serologic responses for HBeAg should be evaluated in persons who are HBeAg positive at baseline to assess whether they lose HBeAg and subsequently develop anti-HBe. This represents an important immunologic milestone in treatment response and can signal greater intrahepatic control of HBV infection. It is also a benchmark for which consideration of treatment cessation can be made – see below for further discussion. The loss of HBeAg and seroconversion to anti-HBe occurs at variable rates with oral antiviral therapy and typically does not occur until virologic response is achieved. Thus, once a virologic response has been achieved, HBeAg and anti-HBe should be assessed every 6 months until HBeAg loss and anti-HBe seroconversion occur.
• HBsAg Loss and anti-HBs Seroconversion: This seroconversion is the ultimate goal of any HBV antiviral therapy, but it is a rare event with oral antiviral therapy (Figure 14).[25,27,28,29,30] Nevertheless, evaluation of HBsAg and anti-HBs should be performed annually for those individuals who have achieved complete HBV viral suppression for more than a year, particularly since it may factor into decisions to stop therapy and assess for sustained off-treatment response. For persons who are HBeAg positive at baseline, monitoring for HBsAg loss and anti-HBs seroconversion should not start until HBeAg/Ab seroconversion has occurred. For persons who are HBeAg negative at baseline, evaluation for HBsAg and anti-HBs can be annually assessed once complete HBV suppression has been maintained for at least one year.
• Quantitative Hepatitis B Surface Antigen (HBsAg): The quantitative HBsAg reflects intrahepatic HBV viral burden that arises from integrated HBV DNA and can be measured via a commercial assay. This can have a role in predicting those who are likely to respond (or not) to peginterferon therapy, as well as those most likely to maintain sustained off-treatment control of their infection after cessation of oral antiviral therapy. The 2025 AASLD/IDSA HBV Treatment Guideline advises against routine use of this test that quantitative HBsAg levels be used when attempting to identify HBeAg-negative individuals with suppressed HBV DNA who may be able to safely stop antiviral therapy. Specifically, the 2025 AASLD/IDSA HBV Treatment Guideline recommends using a cutoff of less than 100 IU/mL as a value that predicts HBsAg loss without the need for retreatment.[6]

Monitoring Treatment Response with Peginterferon Therapy

Peginterferon is recommended for a fixed duration of 48 weeks for chronic HBV infection, regardless of HBeAg status or other clinical factors. Peginterferon does not typically result in comparable on-treatment virologic responses as one might observe with oral antiviral therapy, but in selected patients, it can result in greater gains in control of HBV DNA levels and achieving serologic end points, even after treatment has been completed.[31] For example, in patients who are HBeAg negative, a 1-year course of peginterferon results in a higher rate of HBsAg loss than with the same duration of oral antiviral therapy (4% versus 0 to 1%) despite a lower rate of undetectable HBV DNA (63% versus 90 to 94%).[4] The desired treatment end points for peginterferon include sustained off-treatment HBV DNA less than 2,000 IU/mL, normalization of ALT, HBeAg loss (if HBeAg positive), and ultimately HBsAg loss. Monitoring of HBV DNA levels is recommended at baseline, 3 and 6 months after starting treatment, and at the end of treatment. Among the various biomarkers, quantitative serum HBsAg (qHBsAg) appears to be the strongest early on-treatment predictor of treatment response to peginterferon, and this biomarker has been used to guide early stopping of therapy in patients in Asia and Europe who have been identified as not likely to respond.[1,4] Post-treatment monitoring for treatment response is important since many of the clinical end points noted above, particularly the serologic end points of HBeAg loss and seroconversion and HBsAg loss, may occur months or even years after cessation of peginterferon.

Laboratory Monitoring with Oral Antiviral Therapy

The following table provides a summary overview of baseline monitoring and on-treatment monitoring in persons receiving oral antiviral therapy (Table 1).

Laboratory Monitoring While Receiving Oral Antiviral Therapy

The following table provides a summary overview of baseline monitoring and on-treatment monitoring in persons receiving peginterferon therapy (Table 2).

Oral antiviral agents have emerged as the main therapeutic option for chronic HBV treatment, given the favorable safety profile of these agents and the higher virologic response rates when compared with peginterferon-based therapy, where only an estimated 20 to 30% of patients achieve a sustained off-treatment virologic response.[32] The major oral agents recommended in clinical practice include the nucleoside analogue (entecavir) and the nucleotide analogues (tenofovir DF and tenofovir alafenamide). The rates of functional cure or loss of HBsAg are low, even after years of therapy.[25] The necessity of prolonged therapy over an individual’s lifespan has generated interest in the potential for safe discontinuation of nucleoside/nucleotide analogue (NA) therapy and supervised treatment interruption.

Discontinuation of HBV Antiviral Therapy

The 2025 AASLD/IDSA HBV Treatment Guideline recommends against the routine discontinuation of oral antiviral therapy in persons with chronic HBV unless HBsAg loss occurs.[6] This includes persons without cirrhosis who are HBeAg-negative and have been virally suppressed for 2 or more years on HBV antiviral therapy, citing low rates of HBsAg loss and moderate risk of ALT flares and worsening of liver disease, including decompensation when treatment is discontinued.[6] For individuals who strongly wish to discontinue therapy, the 2025 AASLD/IDSA HBV Treatment Guideline recommends they meet the following criteria:

• Do not have any history of advanced fibrosis or cirrhosis, including no history of hepatic decompensation or HCC.
• Have seroconverted to be HBeAg-negative and anti-HBe-positive (if HBeAg-positive at baseline) for at least 1 year with at least 2 years of persistently undetectable HBV DNA levels.
• Have maintained at least 2 years of HBV DNA viral suppression if they were HBeAg-negative at the start of antiviral therapy.
• Do not have coinfection with HIV or HDV.
• Have a quantitative HBsAg level of less than 100 IU/mL
• Are willing to undergo frequent laboratory and clinical monitoring after cessation of therapy.

Other professional guidelines, including the 2025 EASL Hepatitis B Guidelines and 2015 APASL Hepatitis B Guidelines, have more permissive criteria for treatment discontinuation, recommending that individuals without cirrhosis who seroconvert from HBeAg positive at baseline to anti-HBe positive on therapy and experience sustained HBV suppression can discontinue antiviral therapy after a period of additional treatment, referred to as consolidation therapy.[13,33] Similarly, among individuals without cirrhosis who are HBeAg negative at baseline, EASL and APASL leave open the possibility of treatment discontinuation after a minimum of 2 years (APASL) or 3 years (EASL) of HBV DNA suppression.[13,33]  The 2025 EASL Hepatitis B Guidelines incorporates the use of quantitative HBsAg to guide this decision: patients who are HBeAg negative, have achieved sustained undetectable HBV DNA for at least 3 years, and have low HBsAg levels (less than 100 IU/mL for Asian patients and less than 1,000 IU/mL for others), could stop therapy. [33] Factors associated with viral relapse after NA discontinuation include high baseline HBV DNA level, lower baseline ALT, older age, male sex, and shorter treatment duration.[34] In addition, the rates of off-treatment virologic response in HBeAg-positive patients who have discontinued nucleoside analogues depend on the HBeAg status and time since discontinuation (Figure 15).[16]

Additional Considerations for Treatment Discontinuation

Another clinical scenario where discontinuation of antiviral therapy is considered is in those rare occasions when HBsAg is lost, with or without anti-HBs seroconversion. The 2018 AASLD Hepatitis B Guidance notes the relative lack of evidence to guide treatment decisions for such persons.[1] When oral antiviral therapy is discontinued, patients should be monitored closely (as outlined below), with treatment reinitiated if any of the following criteria are met:[6]

• HBV DNA 10,000 IU/mL or greater regardless of ALT level
• ALT ≥5 x upper limit of normal regardless of HBV DNA level
• Total bilirubin greater than 2.5 mg/dL
• Any clinical symptoms of hepatic decompensation
• Personal wish to restart therapy
• Extrahepatic complications of HBV
• Treatment initiation criteria are newly met (e.g., immune-active disease)

Although it is clear that a rebound of HBV viremia is nearly universal after discontinuation of oral antiviral therapy, some individuals who experience a viral relapse can go on to have mild biochemical flares and then transition to an inactive carrier state with eventual loss of HBsAg.[34,35] If HBV transcriptional activity is silenced sufficiently to enable beneficial reexposure to HBV without the worry of dangerous hepatitis flares, and if HBV reexposure can potentially lead to long-lasting disease remission and even spontaneous HBsAg loss, then discontinuation of oral antiviral therapy may be an important therapeutic strategy in some individuals.[36] The challenge at the moment, and the reason why nucleoside analogue discontinuation is not the norm but the exception at this time, is that we do not have well-established biomarkers or clinical indicators to identify these individuals. It should be noted that due to the risk of morbidity and mortality associated with HBV reactivation, discontinuation of oral antiviral therapy is not recommended in patients with cirrhosis. A few cases of life-threatening viral relapse and deaths have been reported in patients with cirrhosis who stopped oral antiviral therapy.[37,38]

The main reason for laboratory monitoring in patients who have stopped oral antiviral therapy for HBV is to monitor for HBV reactivation and flare in the setting of treatment discontinuation. Persons who stop treatment with either peginterferon or oral NA therapy are at risk for HBV reactivation and associated hepatitis flare. Therefore, these individuals need regular monitoring after stopping therapy, particularly during the first year after treatment discontinuation. Recommended monitoring should include evaluation for HBV DNA level, ALT elevation, seroreversion (e.g., recurrent HBeAg after prior loss), and clinical decompensation, such as the development of ascites. The recommended frequency of monitoring of HBV DNA and ALT is every 1–3 months for the initial 6 months, and then every 3 months for the next 6–12 months. Thereafter, monitoring every 3–6 months is recommended, with the exact frequency depending on the ATL and HBV DNA levels. Similar intensive monitoring is warranted for patients with cirrhosis: at monthly intervals for the first 6 months and then every 3 months thereafter for at least one year. In addition, when it comes to monitoring treatment response to peginterferon, post-treatment monitoring is as important since many of the clinical end points noted above, particularly the serologic end points of HBeAg loss and seroconversion and HBsAg loss, may occur months or even years after cessation of peginterferon.

• Most guidelines recommend monitoring individuals with chronic HBV who do not warrant treatment with a serum ALT and HBV DNA level every 6 months at a minimum, with shorter intervals when ALT elevation is noted.
• Baseline liver fibrosi assessment should be performed in the initial evaluation of people with chronic hepatitis B.
• Screening for HCC, if indicated, should occur regardless of whether the patient is undergoing treatment and should be considered part of the monitoring and care of patients with chronic HBV.
• In addition to a complete blood count and hepatic and renal function panels, a baseline HIV test is indicated, since monotherapy with entecavir, tenofovir alafenamide, or tenofovir DF would be contraindicated in persons with HIV.
• For persons receiving tenofovir DF, monitoring includes serum creatinine, serum phosphorus, and urinalysis to assess urine glucose and proteinuria; the recommended testing interval is at least once annually and more frequently in patients at risk for renal injury or with preexisting renal impairment. Routine laboratory monitoring for lactic acidosis is not necessary for patients on oral NA therapy, but assessment of the anion gap and testing of lactic acid level is warranted in a symptomatic patient with advanced liver disease.
• Adverse events with peginterferon therapy can be diverse and can include flu-like symptoms, fatigue, nausea, diarrhea, anorexia (with or without weight loss), emotional lability, depression, bone marrow suppression, and autoimmune phenomena, such as thyroiditis. All patients receiving peginterferon should be monitored for these complications throughout the entire course of therapy.
• Ongoing monitoring of HBV DNA levels is needed to establish the initial treatment response to oral antiviral therapy and ensure early detection of a virologic breakthrough and treatment failure. This should be measured at baseline, then at 3 and 6 months to assess primary virologic response, which is a decline in HBV DNA of greater than 1 log10 IU/mL.
• Additional counseling on adherence and exploration of barriers to treatment is indicated if a patient does not achieve a primary virologic response on oral antiviral therapy.
• Once HBV viral suppression to undetectable levels has occurred on oral antiviral therapy, HBeAg and anti-HBe can be assessed every 6 months until HBeAg loss and anti-HBe seroconversion have occurred.
• Stopping oral antiviral therapy is generally not recommended unless HBsAg loss has occurred. Patients who stop oral antiviral therapy or peginterferon are at risk for HBV reactivation and an associated hepatitis flare. In cases of HBV treatment discontinuation, patients need regular laboratory and clinical monitoring (typically every 1-3 months), particularly in the first year of stopping therapy.